W. M. Keck Microfabrication Facility
  CSM
MSU PA
MSU
Equipment Resource
 

Micro Raman Spectrograph

HoloProbe Raman Spectrograph

The Center for Sensor Materials maintains a state-of-the-art micro Raman spectrograph consisting of a Kaiser Optical Systems HoloProbe Raman Spectrograph coupled to an Olympus BX-60 optical microscope.

Unlike older spectrograph designs, the HoloProbe uses transmissive (rather than reflective) holographic filters and gratings made by Kaiser Optical Systems, to obtain very high light throughput. This allows the spectrograph to be connected to the microscope through an optical fiber, rather than through free space optics. The use of a single mode fiber produces a diffraction limited laser spot ( < 2 µm) at the sample. By incorporating a motorized stage on the microscope, it is possible to visualize the chemical distribution in a sample.

System Specifications:

  • Excitation wavelengths: 532nm or 633nm
  • Laser spot size: < 2 µm (with 100x objective)
  • Spectral resolution: 5 cm-1 (1 cm-1 with High-Resolution grating)
  • Spectral coverage: ~250 - 4000 cm-1 Raman shift

Example

An optical image and a "Raman Map" of two glass spheres in a polymer film. The spheres are ~40 µ in diameter and the spectra was taken at 20 µ intervals. The signal is an integrated polymer peak, so the spheres appear as "holes" in the raman image of the film.